Microbial DNA extraction and PCR from core samples SSK105556 and SSK105560 from UKGEOS Glasgow Observatory, borehole GGC01
Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent Polymerase Chain Reaction (PCR) from core samples collected and preserved for microbiology (SSK105556 and SSK105560) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. NERC grant SHAPE-UK NE/R018006/1.
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Detailed methodology in attached documents. Core samples processed into powder in sterile conditions. Powdered core then used for DNA extractions in Qiagen PowerSoil Pro DNA Extraction kit. Resulting elutes amplified using Polymerase Chain Reaction (PCR). PCR products visualised using gel electrophoresis.
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