Microbial DNA extraction and PCR from core samples SSK105556 and SSK105560 from UKGEOS Glasgow Observatory, borehole GGC01
Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent Polymerase Chain Reaction (PCR) from core samples collected and preserved for microbiology (SSK105556 and SSK105560) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. NERC grant SHAPE-UK NE/R018006/1.
dataset
:
http://data.bgs.ac.uk/id/dataHolding/13608095
English
Geoscientific information
GEMET - INSPIRE themes, version 1.0:
BGS Thesaurus of Geosciences:
Abrasion
Geomicrobiology
NGDC Deposited Data
Scottish SDI
Sandstone
Shale
Free:
NERC_DDC
-4.2200,
55.8400,
-4.2200,
55.8400
Glasgow [id=1298677]
creation: 2023-08-03
2021-06-01
-
2021-06-30
vector
University of Bristol
Katie Edwards
Wills Memorial Building, Queens Road, Clifton,
Bristol,
BS8 1RJ
email:
not available
Role: originator
British Geological Survey
Enquiries
email:
not available
Role: distributor
British Geological Survey
Enquiries
email:
not available
Role: point of contact
Data Quality
Detailed methodology in attached documents. Core samples processed into powder in sterile conditions. Powdered core then used for DNA extractions in Qiagen PowerSoil Pro DNA Extraction kit. Resulting elutes amplified using Polymerase Chain Reaction (PCR). PCR products visualised using gel electrophoresis.
INSPIRE Implementing rules laying down technical arrangements for the interoperability and harmonisation of Geology
Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
Constraints
The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.
There are no restrictions on the use that may be made of the dataset, although an appropriate copyright acknowledgement must be made when any part of the dataset is reproduced. Either no third party data / information is contained in the dataset or BGS has secured written permission from the owner of any third party data / information contained in the dataset to make the dataset freely available without any use constraints - inclusion of any third party data / information will affect the copyright acknowledgement that needs to be made.
licenceOGL
Metadata about metadata
027b4472-e717-1de8-e063-0937940a5dd1
British Geological Survey
Environmental Science Centre,Keyworth,
NOTTINGHAM,
NG12 5GG,
United Kingdom
tel: +44 115 936 3100
email:
enquiries@bgs.ac.uk
Role: point of contact
2024-04-24