Microbial DNA extraction and PCR from core samples SSK111456 and SSK111457 from UKGEOS Glasgow Observatory borehole GGC01
Loan IDA number IDA271576. Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent PCR from shale and sandstone core samples collected and preserved for microbiology (shale SSK111456 and sandstone SSK111457) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. Samples and data are derived from the UK Geoenergy Observatories Programme funded by the UKRI Natural Environment Research Council and delivered by the British Geological Survey.
dataset
:
http://data.bgs.ac.uk/id/dataHolding/13607775
English
Geoscientific information
GEMET - INSPIRE themes, version 1.0:
BGS Thesaurus of Geosciences:
Scottish SDI
Abrasion
Geomicrobiology
NGDC Deposited Data
Sandstone
Shale
Free:
NERC_DDC
-4.2216,
55.8411,
-4.2216,
55.8411
Glasgow [id=1298677]
creation: 2021-06-22
2020-03-01
-
2020-08-31
text table
University of Bristol
Katie Edwards
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BS8 1RJ
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Role: originator
British Geological Survey
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Data Quality
Detailed methodology in attached documents. Core samples processed into powder in sterile conditions. Powdered core then used for DNA extractions in Qiagen PowerSoil Pro DNA Extraction kit. Resulting elutes amplified using Polymerase Chain Reaction (PCR). PCR products visualised using gel electrophoresis.
INSPIRE Implementing rules laying down technical arrangements for the interoperability and harmonisation of Geology
Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
Constraints
The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.
Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"
licenceOGL
Metadata about metadata
c5f78e0e-2f47-7141-e054-002128a47908
British Geological Survey
Environmental Science Centre,Keyworth,
NOTTINGHAM,
NG12 5GG,
United Kingdom
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2024-04-24