Intra-crystalline protein degradation dating of proboscidean enamel from the UK (NERC Grant NE/K500987/1)
The concentration of both L and D amino acid isomers was determined by HPLC (High-performance liquid chromatography) for multiple amino acids. 30 samples of proboscidean enamel were taken from the collections at BGS. Samples of enamel (~30 mg) was removed from the teeth of proboscideans using a small electric drill. All of the samples were from UK sites dating to the Quaternary. These samples were used to build a regional geochronology based on the extent of racemisation of amino acids from the intra-crystalline fraction (Dickinson, 2018). Thesis http://etheses.whiterose.ac.uk/22261. A published paper 'A new method for enamel amino acid racemization dating: A closed system approach' is also available; https://doi.org/10.1016/j.quageo.2018.11.005
dataset
:
http://data.bgs.ac.uk/id/dataHolding/13607694
English
Geoscientific information
GEMET - INSPIRE themes, version 1.0:
BGS Thesaurus of Geosciences:
NGDC Deposited Data
Age determination methods
Racemization dating
Geochronology
Free:
Free:
NERC_DDC
0.0700,
51.4400,
1.7000,
52.9200
ENG, ENGLAND [id=150000]
creation: 2020-11-27
2017-05-08
-
2018-07-01
text table
University of York
Marc Dickinson
York,
YO10 5DD
email:
not available
Role: originator
British Geological Survey
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NOTTINGHAM,
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British Geological Survey
Enquiries
Environmental Science Centre, Nicker Hill, Keyworth,
NOTTINGHAM,
NG12 5GG,
United Kingdom
tel: 0115 936 3143
email:
enquiries@bgs.ac.uk
Role: point of contact
Data Quality
Samples of enamel (~30 mg) were powdered and exposed to NaOCl (12%) for 72 h. The NaOCl was removed and the powdered enamel was washed 5X with water and 1X with MeOH. Powdered enamel samples were split into 2 fractions. One sample was treated for their free amino acid (FAA) content and the other their total hydrolysable amino acid (THAA) content. THAA samples were dissolved in HCl (7M) and heated at 110 ̊C for 24 h. THAA samples were re-dissolved in HCl (1 M, 20 μLmg−1) and FAA samples were demineralised in HCl (1 M, 25 μLmg−1). KOH (1 M, 28 μLmg−1) was added to the acidified solutions. The sample was centrifuged for 10 min causing a clear supernatant to form above a gel; the biphasic separation. The supernatant was extracted and dried by centrifugal evaporation and analysed by HPLC. All methods are outlined in Dickinson et al., 2019.
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b5a67555-ae07-3623-e054-002128a47908
British Geological Survey
Environmental Science Centre,Keyworth,
NOTTINGHAM,
NG12 5GG,
United Kingdom
tel: +44 115 936 3100
email:
enquiries@bgs.ac.uk
Role: point of contact
2024-04-24