Buffalo Cave Flowstone - Annual Laminae (NERC grant NE/J00443X/1)
Annual lamina thickness (microns), lamina count, and age model (Ma, years) for the Early Pleistocene (Lines 1 and 2) and early Holocene speleothems from Buffalo Cave, South Africa. The samples, methods and results are described in full in: Hopley, P. J. et al. (2018) Orbital precession modulates interannual rainfall variability, as recorded in an Early Pleistocene speleothem. Geology. DOI:10.1130/G45019.1
Speleothems were imaged on a Leica SP2 confocal microscope using an oil immersion objective lens. Individual images of 1024 x 1024 pixels at 2x zoom were acquired over the entire length of the speleothems by 3 microscope fields in the y axis. The Leica LCS software automatically stitched all the image frames to produce a montage of both samples. Laser excitation wavelengths of 488 nm and 515 nm were used to detect the autofluorescence of the organic matter with emission detection set to 540 – 680 nm; pixel resolution was 0.183 µm. Greyscale line profiles were produced for each photomosaic using the image processing software ImageJ and imported into Matlab. The laminae were measured and counted using the automated peak-counting algorithm of Smith et al. (2009). Age models were produced using a combination of magnetostratigraphy, orbital tuning and U-Th dating (see Hopley et al., 2018).
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
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6f9e75e7-993e-620d-e054-002128a47908
British Geological Survey
Environmental Science Centre,Keyworth,
NOTTINGHAM,
NG12 5GG,
United Kingdom