QICS Data: Effect of a controlled sub-seabed release of CO2 on the meiofauna community of shallow marine sediments (2012 - 2013)

Sediment cores were collected from the 4 zones that had different distance to the CO2 injection point. Samples were taken pre-injection, during the gas injection, and during 4 sampling campaigns conducted up to 1 year post-injection. Samples of sediment were taken in 4 zones: Zone 1: Directly above the CO2 injection point Zone 2: 25 m away from the injection point Zone 3: 75 m away from the injection point Zone 4: 450 m away from the injection point Collection of meiofauna samples. Push cores made of Plexiglass, 24 cm long and 5.9 cm internal diameter were used by divers to collect meiofauna samples. Five replicates were taken approximately 1 m apart at each sampling point and station, four of which were used to produce the data. The exact same area sampled at a previous time point was avoided. The samples were preserved in 10% formalin. Extraction and identification of meiofauna. The sample was gently washed with freshwater onto a 63 μm sieve to remove the formalin before it was tipped into a large lidded jar (25cm high, 17 cm diameter). Water was added to within a 4 cm of the top of the jar and the jar was inverted approximately 6 times so the sediment was not sticking to the bottom. The mixture was allowed to settle for 8 seconds before the supernatant was poured through a 63 μm sieve. This was repeated five times and the material on the sieve was tipped into a 500 ml beaker using Ludox TM. To check the efficiency of the elutriation the residues of the first 5 samples residue were collected and checked for nematodes using a dissecting microscope. Ludox TM, a colloidal silica solution with a specific gravity of 1.15 was added to the beaker and the mixture was thoroughly stirred. After being left for one hour the neutrally buoyant meiofauna remained in suspension and the supernatant was poured through a sieve to collect the animals which were then washed with alcohol into a glass vial. Ludox was added to the sample and the process was repeated five times. The residues of the first 5 samples were checked for nematodes. Due to the large number of animals in the samples a proportion of 15% was extracted by washing the sample into a container with freshwater and adding water corresponding to a volume of 20 x 50 ml ladles. The mixture was throughly agitated before 3 ladles of the mixture were removed and collected on a 63 μm sieve. The subsample was then washed into a cavity block using a mixture of 5% glycerol and 20% ethanol plus water. The mixture was allowed to evaporate overnight on a warm hotplate to pure glycerol and then mounted on slides. Nematodes were identified and enumerated, with a compound microscope, to the lowest possible taxonomic level using pictorial keys (Warwick et al. 1998). Warwick RM, Platt HM, Somerfield PJ (1998) Freeliving marine nematodes. Part III. Monhysterids. Synopses of the British Fauna (New Series) No. 53. Field Studies Council, Shrewsbury, UK.

INSPIRE Implementing rules laying down technical arrangements for the interoperability and harmonisation of Geology

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services